Biological impact of lipophilic sediment extracts from the Kafue River, Zambia, in microinjected rainbow trout yolk-sac fry and chick embryo livers exposed in vitro

Authors

  • L. Norrgren Department of Pathology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden
  • B. Brunström Department of Environmental Toxicology, Uppsala University, 752 36 Uppsala, Sweden
  • M. Engwall Department of Environmental Toxicology, Uppsala University, 752 36 Uppsala, Sweden
  • M. Mwase University of Zambia, School of Veterinary Medicine, Department of Paraclinical Studies, Lusaka, Zambia

Keywords:

Morphology, EROD activity, Pesticides

Abstract

Lipophilic sediment extract originating from the Kafue River, Zambia, was evaluated for toxic potency. Two different bioassays were used. In the first, sediment extracts were injected into newly hatched rainbow trout yolk-sac fry, and in the second chick embryo livers were incubated in vitro in different concentrations of the sediment extract. A dose-dependent ethoxyresofurin-O-deethylase (EROD) induction was recorded in both experiments. Furthermore, various types of intracellular alterations characterized by degenerative and necrotic changes were observed in the hepatocytes of the exposed groups. These findings indicate the presence of EROD inducing dioxin-like lipophilic substances in sediment extracts originating from the Kafue River near the town of Kitwe.

References

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Published

1998-01-01

Issue

Vol. 1 No. 1 (1998): Aquatic Ecosystem Health & Management

Section

Research article

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