Bioassays for testing effects of Al, Cr and Cd using development in the amphibian Pleurodeles waltl and regeneration in the planarian Dugesia etrusca
Keywords:
Heavy metals, ToxicologyAbstract
Historically, water quality studies have been directed toward obtaining physical and chemical measurements on toxicants occurring in the aquatic environment. At present, bioassays are increasingly used as sensitive indicators of pollutant toxicity, since they are rapid, inexpensive, applicable to a variety of toxicants and allow several acute and chronic endpoints to be assessed simultaneously. The analysis of the potential toxicity of heavy metals was conducted on two aquatic model systems, an amphibian, Pleurodeles waltl, and a freshwater planarian, Dugesia etrusca. Aluminum, chromium and cadmium were chosen since human activity has led to a sharp increase of these metals in the environment. Environmentally realistic and supra-environmental concentrations were assayed in short-term toxicity tests. The effects of these metal ions were evaluated by the analysis of both malformations and lethality in amphibian embryos and alterations to planarian regeneration. Aluminum (0.15, 0.75 and 1.5 mmol 1-1) and Cr (0.75 and 1.5 mmol 1-1) were toxic to P. waltl development and both the ions affected planarian regeneration at concentrations ranging from 0.25 to 1.5 mmol 1-1. Cadmium was highly toxic to amphibian embryos at concentrations ranging from 0.18 to 50 μmol 1-1. This metal ion was also highly toxic to planarians, but it did not significantly affect the regeneration process. The results indicate that bioassays with these two organisms are useful to assess the toxic potential of aquatic pollutants.
References
Anderson, S.L. and Wild, G.C. 1994. Linking genotoxic responses and reproductive success in ecotoxicology. Environ. Health Perspect., 102: 9–12.
Anderson, S.L., Sadinski, W., Shugart, L., Brussard, P., Depledge, M., Ford, T., Hose, J., Stegeman, J., Suk, W., Wirgin, I. and Wogan, G. 1994. Genetic and molecular ecotoxicology: a research framework. Environ. Health Perspect., 102: 3–8.
Appanna, V.D., Gazsó, L.G., Huang, J. and St. Pierre, M. 1996. Mechanisms of detoxification in Pseudomonas fluorescens is dependent on iron. Bull. Environ. Contam. Toxicol., 57: 875–880.
Best, J.B. and Morita, M. 1982. Planarians as a model system for in vitro teratogenesis studies. Teratogen. Carcinogen. Mutagen., 2: 277–291.
Beyersmann, D. 1995. “Physiochemical aspects of the interference of detrimental metal ions with normal metal metabolism”. In Handbook of Metal-Ligand Interactions in Biological Fluids, Edited by: Berthon, G. 1–20. New York: Marcel Dekker. In:
Blaustein, A.R., Wake, D.B. and Sousa, W.P. 1994. Amphibian declines: judging stability, persistence, and susceptibility of populations to local and global extinctions. Conserv. Biol., 8: 60–71.
Bowen, H.J.M. 1979. Environmental Chemistry of the Elements, New York: Academic Press.
Dally, H. and Hartwig, A. 1997. Induction and repair inhibition of oxidative DNA damage by nickel and cadmium in mammalian cells. Carcinogenesis, 18: 1021–1026.
Dawson, D.A. and Bantle, J.A. 1987. Development of a reconstituted water medium and preliminary validation of the frog embryo teratogenesis assay-Xenopus (Fetax). J. Appl. Toxicol., 7: 237–244.
Dawson, D.A., McCormick, C.A. and Bantle, J.A. 1985. Detection of teratogenic substances in acidic mine water samples using the frog embryos teratogenesis assay-Xenopus (FETAX). J. Appl. Toxicol., 5: 234–244.
Dawson, D.A., Steble, E., Burks, S.A. and Bantle, J.A. 1988. Evaluation of the developmental toxicity of metal-contaminated sediments using short-term minnow and frog embryo-larval assays. Environ. Toxicol. Chem., 7: 27–34.
De, S.K., Dey, S.K. and Andrews, G.K. 1990. Cadmium teratogenicity and its relationship with metallothionein gene expression in midgestation mouse embryos. Toxicology, 64: 89–104.
DeYoung, D.J., Bantle, J.A., Hull, M.A. and Burks, S.L. 1996. Differences in sensitivity to developmental toxicants seen in Xenopus and Pimephales embryos. Bull. Environ. Contam. Toxicol., 56: 143–150.
Elinder, C.-G. and Järup, L. 1996. Cadmium exposure and health risks: recent findings. Ambio, 25: 370–373.
Gallien, L. and Durocher, M. 1957. Table chronologique du développement chez Pleurodeles waltl (Michah). Bull. Biol. Fr. Belg., 91: 97–114.
Guttman, S.I. 1994. Population genetic structure and ecotoxicology. Environ. Health Perspect., 102: 97–100.
Hartwig, A. 1995. Current aspects in metal genotoxicity. BioMetals, 8: 3–11.
Hasnain, S. and Sabri, A.N. 1997. Growth stimulation of Triticum aestivum seedlings under Cr-stresses by non rhizospheric pseudomonad strains. Environ. Pollut., 97: 265–273.
Hassoun, E.A. and Stohs, S.J. 1995. Chromium-induced production of reactive oxygen species, DNA single-strand breaks, nitric oxide production, and lactate dehydrogenase leakage in J774A.1 cell cultures. J. Biochem. Toxicol., 10: 315–321.
Henry, T.R., Spitsbergen, J.M., Hornung, M.W., Abnet, C.C. and Peterson, R.E. 1997. Early life stage toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in zebrafish (Danio rerio). Toxicol. Appl. Pharmacol., 142: 56–68.
Imai, A. and Gloyna, E.F. 1996. Speciation of chromium(III) in activated sludge. Wat. Environ. Res., 68: 301–310.
IARC (International Agency of Research on Cancer). 1993. Beryllium, Cadmium, Mercury, and Exposures in the Glass Manufacturing Industry, Lyon: Monographs on the Evaluation of the Carcinogenic Risks to Humans, IARC.
Jaylet, A., Gauthier, L. and Fernandez, M. 1987. Detection of mutagenicity in drinking water using a micronucleus test in newt larvae (Pleurodeles waltl). Mutagenesis, 2: 211–214.
Kumar, R., Agarwal, A.K. and Seth, P.K. 1996. Oxidative stress-mediated neurotoxicity of cadmium. Toxicol. Lett., 89: 65–69.
Mahaney, P.A. 1994. Effects of freshwater petroleum contamination on amphibian hatching and metamorphosis. Environ. Toxicol. Chem., 13: 259–265.
Nair, S. and Krishnamurthi, V.S. 1991. Effect of Cr on growth of Pseudomonas aeruginosa. Indian J. Exp. Biol., 29: 140–144.
Ono, A., Sekita, K., Ogawa, Y., Hirose, A., Suzuki, S., Saito, M., Naito, K., Kaneko, T., Furuya, T., Kawashima, K., Yasuhara, K., Matsumoto, K., Tanaka, S., Inoue, T. and Kurokawa, Y. 1996. Reproductive and developmental toxicity. Studies of toluene. J. Environ. Path. Toxicol. Oncol., 15: 9–20.
Palmer, A.K. 1993. Identifying environmental factors harmful to reproduction. Environ. Health Perspect., 101: 19–25.
Peters, J.M., Duncan, J.R., Wiley, L.M. and Keen, C.L. 1995. Influence of antioxidants on cadmium toxicity of mouse preimplantation embryos in vitro. Toxicology, 99: 11–18.
Plowman, M.C., Grbac-Ivankovic, S., Martin, J., Hopfer, S.M. and Sunderman, F.W. Jr. 1994. Malformations persist after metamorphosis of Xenopus laevis tadpoles exposed to Ni2+, Co2+ or Cd2+ in FETAX assays. Teratogen. Carcinogen. Mutagen., 14: 135–144.
Rugh, R. 1962. The amphibia, 31–31. Minneapolis: Experimental Embryology, Burgess.
Sabourin, T.D., Faulk, R.T. and Goss, L.B. 1985. The efficacy of three non-mammalian test systems in the identification of chemical teratogens. J. Appl. Toxicol., 5: 227–233.
Shi, X. and Dalal, N.S. 1994. Generation of hydroxyl radical by chromate in biologically relevant systems: role of Cr(V) complexes versus tetraperoxochromate(V). Environ. Health Perspect., 102: 231–236.
Snowden, K.C. and Gardner, R.C. 1993. Five genes induced by aluminum in wheat (Triticum aestivum L.) roots. Plant Physiol., 103: 855–861.
Steward, J.W. 1969. The Tailed Amphibians of Europe, Newton Abbot: David and Charles.
Sunderman, F.W. Jr., Plowman, M.C. and Hopfer, S.M. 1991. Embryotoxicity and teratogenicity of cadmium chloride in Xenopus laevis, assayed by the FETAX procedure. Ann. Clin. Lab. Sci., 21: 381–391.
Temara, A., Ledent, G., Warnau, M., Paucot, H., Jangoux, M. and Dubois, P. 1996. Experimental cadmium contamination of Asterias rubens (Echinodermata). Mar. Ecol. Prog. Ser., 140: 83–90.
Waalkes, M.P., Cherian, M.G. and Goyer, R.A. 1992. Immunohistochemical evidence of high concentrations of metallothionein in pancreatic hepatocytes induced by cadmium in rats. Toxicol. Pathol., 20: 323–326.
Weis, J.S. and Weis, P. 1987. Pollutants as developmental toxicants in aquatic organisms. Environ. health Perspect., 71: 77–85.
Witters, H.E., Van Puymbroeck, S., Stouthart, A.J.H.X. and Bonga, S.E.W. 1996. Physicochemical changes of Al in mixing zones: mortality and physiological disturbances in brown trout (Salmo trutta L.). Environ. Toxicol. Chem., 15: 986–996.
WHO (World Health Organization). 1992. Cadmium environmental aspects: toxicity to aquatic organisms, Environ. Health Criteria, Geneva: WHO.
Wong, K.L. and Klaassen, C.D. 1982. Neurotoxic effects of cadmium in young rats. Toxicol. Appl. Pharmacol., 63: 330–337.
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