Mutagenicity of bleached and unbleached effluents from Baikalsk pulp and paper mill at Lake Baikal, Russia

Authors

  • S.V. Kotelevtsev Biological Department, Laboratory of Physical Chemistry of Biomembranes, Moscow State University, 119899 Moscow, Russian Federation
  • O.O.P. Hänninen Department of Physiology, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland
  • P.A. Lindström-Seppä Department of Physiology, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland
  • S.E. Huuskonen Department of Physiology, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland
  • L.I. Stepanova Biological Department, Laboratory of Physical Chemistry of Biomembranes, Moscow State University, 119899 Moscow, Russian Federation
  • V.M. Glaser Biological Department, Laboratory of Physical Chemistry of Biomembranes, Moscow State University, 119899 Moscow, Russian Federation
  • A.M. Beim Institute of Ecological Toxicology, Baikalsk, P.O. Box 48, 665914 Irkutsk region, Russian Federation

Keywords:

Fish, Cytochrome P-4501A, Induction

Abstract

The mutagenicity of bleached pulp mill effluents was compared to the mutagenicity of unbleached waste waters. Mutagenicity was assessed with the Ames test, using metabolic activation systems isolated from the liver of rats and fish. Liver extracts from fish caught in polluted areas, and from fish experimentally exposed to waste water, were also investigated. Pulp mill effluents taken after chlorination showed mutagenic activity. The activity diminished during the waste water treatment. Tissue extracts from fish exposed to various concentrations of treated bleached and unbleached pulp mill effluents showed only slight mutagenic activity in a few samples. In the case of bleached pulp mill effluents monooxygenase activities were elevated in those samples where slight mutagenicity was observed. In the case of unbleached effluents no such correlation was found.

References

Ames, B.N., McCann, J. and Yamasaki, E. 1975. Methods for detecting carcinogens and mutagen with the Salmonella/mammalian microsome mutagenicity test. Mutat. Res., 31: 347–364.

Ander, P., Eriksson, K-E., Kolar, M-C., Kringstad, K., Rannung, U. and Ramel, C. 1977. Studies on the mutagenic properties of bleaching effluent. Sven. Papperstidn., 80: 454–459.

Bagnasco, M., Camoirano, A., De Flora, S., Melodia, F. and Arillo, A. 1991. Enhanced liver metabolism of mutagens and carcinogens in fish living in polluted seawater. Mutat. Res., 262: 129–137.

Bjørseth, A., Carlberg, G.E. and Møller, M. 1979. Determination of halogenated organic compounds and mutagenicity testing of spent bleach liquors. Sci. Tot. Environ., 11: 197–211.

Burke, M.D. and Mayer, R.T. 1974. Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab. Dispos., 2: 583–588.

Elskus, A.A., Stegeman, J.J., Susani, L.C., Black, D., Pruell, R.J. and Fluck, S.J. 1989. Polychlorinated biphenyls concentration and cytochrome P-450E expression in winter flounder from contaminated environments. Mar. Environ. Res., 28: 25–30.

Förlin, L. and Hansson, T. 1982. Effects of treated municipal wastewater on hepatic, xenobiotic and steroid metabolism in trout. Ecotoxicol. Environ. Saf., 6: 41–48.

Glaser, V.M., Kotelevtsev, S.V., Stepanova, L.I., Abilev, S.K., Buevich, G.V. and Beim, A.M. 1990. The assessment of mutagenicity in the Ames test of sewage waters and industrial effluent of the Baikal cellulose paper integrated plan. Biologicheskie nauki, 1: 101–109. (in Russian)

Gooch, J.W., Elskus, A.A., KloepperSams, P.J., Hahn, M.E. and Stegeman, J.J. 1989. Effects of ortho- and non-ortho-substituted polychlorinated biphenyl congeners on the hepatic mono-oxygenase system in scup (Stenotomus chrysops). Toxicol. Appl. Pharmacol., 98: 422–433.

Höglund, C., Allard, A.-S., Neilson, A.H. and Landner, L. 1979. Is the mutagenic activity of bleach plant effluents persistent in the environment?. Sven. Papperstidn., 15: 447–449.

Holmbom, B., Voss, R.H., Mortimer, R.D. and Wong, A. 1984. Fractionation, isolation and characterization of Ames mutagenic compounds in kraft chlorination effluents. Environ. Sci. Technol., 18: 333–337.

Kinae, N., Yamashita, M., Daicho, N., Tomita, I. and Kimura, I. 1988. Mutagenicity of pulp and paper mill effluents and their tumor-inducing effect on catfish (Plotosus anguillaris). Mutat. Res., 203: 376–376.

Klotz, A.V., Stegeman, J.J. and Walsh, C. 1984. An alternative 7-ethoxyresorufin O-deethylase activity assay; a continuous visible spectrophotometric method for measurement of cytochrome P-450 monooxygenase activity. Anal. Biochem., 140: 138–145.

Kotelevtsev, S.V., Kozlov, Y.P. and Stepanova, L.I. 1986. Ecotoxicological control of environment with physico-chemical methods. Biologicheskie nauki, 1: 19–30. (in Russian)

Kukkonen, J. 1992. Effects of lignin and chlorolignin in pulp mill effluents on the binding and bioavailability of hydrophobic organic pollutants. Water Res., 26: 1523–1532.

Liimatainen, A., Müller, D., Vartiainen, T., Jahn, F., Kleeberg, U., Klinger, W. and Hänninen, O. 1988. Chlorinated drinking water is mutagenic and causes 3-methylcholanthrene type induction of hepatic monooxygenase. Toxicology, 51: 281–289.

Lindström-Seppä, P. and Oikari, A. 1989. Biotransformation and other physiological responses in whitefish caged in a lake receiving pulp and paper mill effluents. Ecotoxicol. Environ. Saf., 18: 191–203.

Lindström-Seppä, P. and Oikari, A. 1990. Biotransformation and other toxicological and physiological responses in rainbow trout (Salmo gairdneri Richardson) caged in a lake receiving effluents of pulp and paper industry. Aquat. Toxicol., 16: 187–204.

Lindström-Seppä, P., Huuskonen, S., Pesonen, M., Muona, P. and Hänninen, O. 1992. Unbleached pulp mill effluents affect cytochrome P-450 monooxygenase enzyme activities. Mar. Environ. Res., 34: 157–161.

Lowry, O.H., Rosebroug, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265–275.

McKague, A.B., Lee, E.G-H. and Douglas, G.R. 1981. Chloroacetones: Mutagenic constituents of bleached kraft chlorination effluent. Mutat. Res., 19: 301–306.

McLeay, D.J. 1987. Aquatic toxicology of pulp and paper mill effluent: a review EPS 4/PF/1, Ottawa, Ontario: Environment Canada.

Nebert, D.W. and Gelboin, H.V. 1968. Substrate inducible microsomal arylhydrocarbon hydroxylase. II. Cellular responses during enzyme induction. J. Biol. Chem., 244: 6242–6249.

Payne, J.F., Fancey, L.L., Rahimtula, A.D. and Porter, E.L. 1987. Review and perspective on the use of mixed-function oxygenase enzymes in biological monitoring. Comp. Biochem. Physiol, 86C: 233–245.

Pesonen, M. and Andersson, T. 1992. Toxic effects of bleached and unbleached paper mill effluents in primary cultures of rainbow trout hepatocytes. Ecotoxicol. Environ. Saf., 24: 63–71.

Rannung, U., Jenssen, D., Ramel, C., Eriksson, K-E. and Kringstad, K. 1981. Mutagenic effects of effluents from chlorine bleaching of pulp. J. Toxicol. Environ. Health, 7: 33–47.

Stegeman, J.J., Teng, F.Y. and Snowberger, E.A. 1987. Induced cytochrome P-450 in winter flounder (Pseudopleuronectes americanus) from coastal Massachusetts evaluated by catalytic assay and monoclonal antibody probes. Can. J. Fish. Aquat. Sci., 44: 1270–1277.

Swanson, S.E., Rappe, C., Malmström, J. and Kringstad, K.P. 1988. Emissions of PCDDs and PCDFs from the pulp industry. Chemosphere, 17: 681–691.

Ullrich, V. and Weber, P. 1972. The O-dealkylation of 7-ethoxycoumarin by liver microsome: a direct fluorometric test, Hoppe-Seyler's. Z. Physiol. Chem., 353: 1171–1177.

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Published

2000-01-01

Issue

Vol. 3 No. 1 (2000): Large Lakes of the World: Comparative Ecology

Section

Research article

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