Monooxygenase activities in the liver of fish living in Lake Baikal and after treatment with waste waters from the Baikalsk Pulp and Paper Mill

Authors

  • S.V. Kotelevtsev Department of Biology, Moscow State University, 119899 Moscow, Russia
  • L.I. Stepanova Department of Biology, Moscow State University, 119899 Moscow, Russia
  • P. Lindström-Seppä Department of Physiology, University of Kuopio, P.O. Box. 1627, FIN-70211 Kuopio, Finland
  • O. Hänninen Department of Physiology, University of Kuopio, P.O. Box. 1627, FIN-70211 Kuopio, Finland

Keywords:

Bleached kraft, Grayling, Sculpin

Abstract

The biological effects of the waste waters released by Baikalsk Pulp and Paper Mill were studied in fish collected from Lake Baikal. Fish were also exposed in laboratory conditions to known inducers of hepatic monooxygenase activities. We did not find any differences in monooxygenase activities of grayling and sculpins caught near the pulp and paper mill compared to control fish caught in the middle part of Lake Baikal. The results showed that the waste water treatment in the mill is effective. Usually the treated waste water enters the lake diluted up to 5% concentration. However, only at 20% concentration did the treated waste water induce hepatic monooxygenase activities in the fish studied in laboratory conditions.

References

Andersson, T.B., Bengson, E., Förlin, L., Härdig, J. and Larson, A. 1987. Long term effects of bleached kraft mill effluents on carbohydrate metabolism and hepatic xenobiotic biotransformation enzymes in fish. Ecotoxicol. Environ. Safety, 13(1): 53–60.

Grachev, M.A., Slobodyanyuk, S.J., Kholodilov, N.G., Fyodorov, S.I., Belikov, S.I., Sherbakov, D.Yu., Sideleva, V.G., Zubin, A.A. and Kharchenco, V.V. 1992. Comparative study of two protein-coding regions of mitochondrial DNA from three endemic sculpins (Cottoidei) of Lake Baikal. J. Mol. Evol., 34(1): 85–90.

Johannesen, K.A.M. and de Pierre, J.W. 1978. Measurement of cytochrome P-450 in the presence of large amounts of contaminating haemoglobin and methemoglobin. Anal. Biochem., 86(2): 725–732.

Kozhova, O.M. and Beim, A.M. 1993. Ekologicheskij monitoring Baikala (Ecological monitoring of Baikal Lake), Moscow, , Russia: Ekologija Ltd. (in Russian)

Kotelevtsev, S.V., Stvolinskyi, S.L. and Beim, A.M. 1986. Ekotoksicologicheskiy Analiz na Osnove Biologicheskih Membran (Ecotoxicological Analysis on Base of the Biological Membrane), Edited by: Boldyrev, A.A. Moscow: Moscow State University. (in Russian)

Kucklick, J.R., Harvey, H.R., Ostrom, P.H., Ostrom, N.E. and Baker, J.E. 1996. Organochlorine dynamics in the pelagic food web of Lake Baikal. Environ. Toxicol. Chem., 15(8): 1388–1400.

Lindström-Seppä, P. and Oikari, A. 1990. Biotransformation activities of feral fish in waters receiving bleached pulp mill effluents. Environ. Toxicol. Chem., 9(11): 1415–1424.

Prough, R.A., Burke, M.D. and Mayer, R.T. 1978. “Direct fluorimetric methods for measuring mixed-function oxidase activity”. In Methods of Enzymology, Edited by: Colowick, S.P. and Kaplas, N.O. 372–377. New York: Academic Press. In

Roy, S. and Hänninen, O. 1994. “Biochemical monitoring of the aquatic environment: possibilities and limitation”. In Ecotoxicology Monitoring, Edited by: Richardson, M. 119–135. New York: VCH. In

Soimasuo, R., Jokinen, I., Kukkonen, J., Petänen, T., Ristola, T. and Oikari, A. 1995. Biomarker responses along a pollution gradient: effects of pulp and paper mill effluents on gaded whitefish. Aquat. Toxicol., 31: 329–345.

Ullrich, V. and Weber, P. 1972. O-deethylation of 7-ethoxycoumarin by liver microsomes. Hopper-Seyler's Z. Physiol. Chem., 353(7): 1171–1177.

Published

2000-01-01