Induction of ethoxyresorufin-O-deethylase (EROD) activity in the liver of black porgy (Acanthopagrus schlegeli) exposed to benzo(a)pyrene
Keywords:
PAH, depuration experiment, kinetics processAbstract
This paper studied the induction kinetics of the enzyme 7-ethoxyresorufin O-deethylase in the liver of the marine fish, Acanthopagrus schlegeli following the exposure to benzo (a)pyrene for up to 14 days at concentrations of 0.5, 1.0, 2.0,5.0 μg l−1 . The results demonstrated a dose-response relationship between hepatic enzyme activities induction and benzo(a)pyrene concentration. Hepatic enzyme activities were significantly induced by the second day with exposure to the lower concentrations of benzo(a)pyrene (0.5 μg l−1 and 1.0 μ g l−1 ), and at 12 hours of exposure for the higher concentrations (2.0 l−1 and 5.0 μ g l−1 ). After 7 days depuration, the activities of the enzyme of fish returned to the control level. This indicated that while benzo(a)pyrene has an effect on the hepatic enzyme activity, the fish can eliminate a major part of BaP metabolites.
References
Aas, E., Beyer, J. and Goksøyr, A. 1998. PAH in fish bile detected by fixed wavelength fluorescence. Mar. Environ. Res., 46: 225–228. [CSA]
Arinç, Ë. and Sen, A. 1999. Hepatic cytochrome P4501A and 7-ethoxyresorufin O-deethylase induction in mullet and common sole as an indicator of toxic organic pollution in Izmir Bay, Turkey. Mar. Environm. Res., 48: 147–160. [CSA][CROSSREF]
Au, D. W. T., Wu, R. S. S., Zhou, B. S. and Lam, P. K. S. 1999. Relationship between ultrastructural changes and EROD activities in liver of fish exposed to benzo[a]pyrene. Environm. Pollu., 104: 235–247. [CSA][CROSSREF]
Britvić, S., Lucić, D. and Kurelec, B. 1993. Bile fluorescence and some early biological effects in fish as indicators of pollution by xenobiotics. Environ. Toxicol. Chem., 12: 765–773. [CSA]
Devaux, A., Flammarion, P., Bernardon, V., Garric, J. and Monod, G. 1998. Monitoring of the chemical pollution of the River Rhône through measurement of DNA damage and cytochrome P4501A induction in chub (Leuciscus cephalus). Mar. Environ. Res., 46(1–5): 257–262. [CSA][CROSSREF]
Fenet, H., Casellas, C. and Bontoux, J. 1998. Laboratory and field-caging studies on hepatic enzymatic activities in European eel and rainbow trout. Ecotoxicol. Environ. Saf., 40: 137–143. [PUBMED][INFOTRIEVE][CSA][CROSSREF]
Gagnon, M. M. and Holdway, D. A. 2000. EROD induction and biliary metabolite excretion following exposure to the water accommodated fraction of crude oil and to chemically dispersed crude oil. Arch. Environm. Contam. Toxicol., 38: 70–77. [CSA][CROSSREF]
Gagnon, M. M. and Holdway, D. A. 2002. EROD activity, serum SDH and PAH biliary metabolites in sandflathead (Platycephalus bassensis) collected in Port Phillip Bay Australia. Mar. Pollu. Bull., 44: 230–237. [CSA][CROSSREF]
Goksøyr, A. and Husøy, A. M. 1998. “Immunochemical approaches to studies of CYP1A localization and induction by xenobiotics in fish”. In Fish Ecotoxicology, Edited by: Braunbeck, T., Hinton, D. E. and Streit, B. 165–202. Basel: Birkha” user Verlag.
Gravato, C. and Santos, M. A. 2002. Juvenile Sea Bass liver P450, EROD induction, and erythrocytic genotoxic responses to PAH and PAH-like compounds. Ecotoxicol. Environm. Saf., 51: 115–127. [CSA][CROSSREF]
Haasch, ML., Prince, R., Wejksnora, P. J., Cooper, K. R. and Lech, J. J. 1993. Caged and wild fish: induction of hepatic cytochrome P-450 (CYP1A1) as an environmental biomonitor. Environm. Toxicol. Chem., 12(6): 885–895. [CSA]
Hawkins, W. E., Walker, W. W., Overstreet, R. M., Lytle, T. F. and Lytle, J. 1988. Dose-related carcinogenic effects of water-borne benzo(a)pyrene on livers of two small fish species. Ecotoxicol. Environm. Saf., 16: 219–231. [CSA][CROSSREF]
Hendricks, J. D., Meyers, T. R., Shelton, T. R., Casteel, J. L. and Bailey, G. S. 1985. Hepatocarcinogenity of benzo(a)pyrene to rainbow trout by dietary exposure and intraperitoneal injection. J. Natl. Cancer Inst., 74: 839–851. [PUBMED][INFOTRIEVE][CSA]
Hodson, P. V., Efler, S., Wilson, J. Y., El-Shaarawi, A., Maj, M. and Williams, T. G. 1996. Measuring the potency of pulp mill effluents for induction of hepatic mixed -function oxygenase activity in fish. J. Toxicol. Environm. Health, 49: 83–110. [CSA][CROSSREF]
Holdway, D. A., Brennan, S. E. and Ahokas, J. T. 2000. Use of hepatic MFO and blood enzyme biomarkers in sand flathead (Platycephalus bassensis) as indicators of pollution in Port Phillip Bay, Australia. Mar. Pollut. Bull., 26: 683–695. [CSA]
Jaksic, Z., Bihari, N., Muller, W. E. G., Zahn, R. K. and Batel, R. 1998. Modulation of cytochrome P450 in sea bass liver by model substances and seawater extracts. Aquat. Toxicol., 40: 265–273. [CSA][CROSSREF]
Kirby, M. F., Neall, P. and Tylor, T. 1999. EROD activity measured in flatfish from the area of the Sea Empress oil spill. Chemosphere, 38(12): 2929–2949. [PUBMED][INFOTRIEVE][CSA][CROSSREF]
Lemaire, P., Mathieu, A., Carriere, S., Narbonne, J -F., Lafaurie, M. and Giudicelli, J. 1992. Hepatic biotransformation enzymes in aquaculture European sea bass (Dicentrarchus labrax): kinetic parameter and induction with benzo(a)pyrene. Comp. Biochem. Physiol., 103(4): 847–853. [CSA][CROSSREF]
Lemaire, P., Forlin, L. and Livingstone, D. R. 1996. Responses of hepatic biotransformation and antioxidant enzymes to CYP1A-inducers (3-methylcholanthrene, b-naphthoavone) in sea bass (Dicentrarchus labrax), dab (Limanda limanda) and rainbow trout (Oncorhynchus mykiss). Aquat. Toxicol., 36: 141–160. [CSA][CROSSREF]
Lemaire-Gony, S. and Lemaire, P. 1992. Interactive effects of cadmium and enzo(a)pyrene on cellular structure and biotransformation enzymes of the liver of the European eel Anguilla anguilla. Aquat. Toxicol., 22: 145–160. [CSA][CROSSREF]
Leonard, J. D. and Hellou, J. 2001. Separation and characterization of gallbladder bile metabolites from speckled trout, Salvelinus fontinalis, exposed to individual polycyclic aromatic compounds. Environm. Toxicol. Chem., 20: 618–623. [CSA][CROSSREF]
Livingstone, D. R. 1993. Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment. J. Chem. Technol. Biotechnol., 57: 195–211. [CSA]
Livingstone, D. R., Mitchelmore, C. L., Peters, L. D., O'Hara, S. C. M. and Shaw, J. P. 2000. Development of hepatic CYP1A and blood vitellogeninin eel (Anguilla anguilla) for use as biomarkers in the Thames Estuary. UK Mar. Environ. Res., 50: 367–371. [CSA][CROSSREF]
Lopez-Barea, J. 1995. “Biomarkers in ecotoxicology: an overview”. In Toxicology in Transition, 1994, E; RO1OX Proceedings Edited by: Degen, G. H., Seiler, J. P. and Bentley, P. 2759–2764. Berlin: Springer-Verlag.
Lopez-Barea, J. and Puyeo, C. 1998. Mutagen content and metabolic activation of promutagens by molluscs as biomarkers of marine pollution. Mutat. Res., 399: 3–15. [PUBMED][INFOTRIEVE][CSA]
MacRae, J. and Hall, K. 1998. Biodegradation of polycyclic aromatic hydrocarbons (PAH) in marine sediment under denitrifying conditions. Wat. Sci. Technol., 38: 177–185. [CSA][CROSSREF]
Maskaoui, K., Zhou, J. L., Hong, H. S. and Zhang, Z. 2002. Contamination by polycyclic aromatic hydrocarbons in the Jiulong river estuary and western Xiamen sea, China. Environm. Pollu., 118: 109–122. [CSA][CROSSREF]
Monteiro, P. R. R., Reis-Henriques, M. A. and Coimbra, J. 2000. Polycyclic aromatic hydrocarbons inhibit in vitro ovarian steroidogenesis in the flounder (Platichthys flesus L.). Aquat. Toxicol., 48: 549–559. [CSA][CROSSREF]
Pacheco, M. and Santos, M. A. 1997. Induction of EROD activity and genotoxicity elects by polycyclic aromatic hydrocarbons and resin acids on the juvenile eel (Anguilla anguilla L.). Ecotoxicol. Environm. Saf., 38: 252–259. [CSA][CROSSREF]
Pacheco, M. and Santos, M. A. 1998. Induction of liver EROD and erythrocytic nuclear abnormalities by cyclophosphamide and PAHs in Anguilla anguilla L. Ecotoxicol. Environm. Saf., 40: 71–76. [CSA][CROSSREF]
Pacheco, M. and Santos, M. A. 1999. Biochemical and genotoxic responses of adult eel (Anguilla anguilla L.) to resin acids and pulp mill effluent: laboratory and field experiments. Ecotoxicol. Environ. Saf., 42: 81–93. [PUBMED][INFOTRIEVE][CSA][CROSSREF]
Raza, H., Otaiba, A. and Montague, W. 1995. H-naphthoflavone-inducible cytochrome P4501A1activity in liver microsomes of the marine safi fish (Siganus canaliculatus). Biochem. Pharmacol., 50(99): 1401–1406. [PUBMED][INFOTRIEVE][CSA][CROSSREF]
Van der Oost, R., Opperhuizen, A., Satumalay, K., Heida, H. and Vermeulen, N. P. E. 1996. Biomonitoring aquatic pollution with feral eel (Anguilla anguilla): I. Bioaccumulation: biota-sediment ratios of PCBs, OCPs, PCDDs and PCDFs. Aquat. Toxicol., 35: 21–46. [CSA][CROSSREF]
Wang, C. G., Chen, J. X., Yu, A. and Zheng, W. Y. 2003. EROD activities of liver in Mugil so-iuy exposed to benzo(a)pyrene, pyrene and their mixture. Acta oceanologica sinica, 22(2): 213–220. [CSA]
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