Physiological responses of Chaetoceros sp. and Nannochloropsis sp. to short-term 2, 4-D, dimethylamine and endosulfan exposure

Authors

  • Yii Siang Hii Faculty of Agrotechnology and Food Science, University Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
  • Kwee Luan Shia Faculty of Agrotechnology and Food Science, University Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
  • Tse Seng Chuah Faculty of Agrotechnology and Food Science, University Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
  • Lee Siang Hing Faculty of Maritime Studies and Marine Science, University Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia

Keywords:

Microalgae, pesticides, primary productivity, short-term toxicity

Abstract

The effects of two widely used pesticides in paddy fields, 2, 4-D, dimethylamine and endosulfan, on growth, photosynthetic rate and the photosynthetic pigments of Chaetoceros sp. and Nannochloropsis sp. were assessed in this study. The 48 hour 50% inhibitory concentration (IC50) value for 2, 4-D dimethylamine on Chaetoceros sp. and Nannochloropsis sp. were 142.2 mg l−1 and 211.8 mg l−1, respectively, and the 48 hour IC50 value for endosulfan on Chaetoceros sp. and Nannochloropsis sp. were 21.9 μ g l−1 and 45.8 μ g l−1, respectively. Endosulfan was much more toxic to the microalgae than 2, 4-D dimethylamine. Both pesticides reduced photosynthetic pigments and oxygen production rate of the microalgae. After 48 hours exposure to the pesticides, the microalgae were transferred to a pesticide-free medium to investigate the post-exposure effect. 2, 4-D dimethylamine and endosulfan did not cause irreversible damage on the microalgae. However, there was a prolonged lag phase and the maximum specific growth rate (μ) of the microalgae was significantly retarded.

References

Ahmad, A., Jamaludin, I., Mahyam, M. I. and Solahuddin, A. R. 2002. “Distribution, Abundance and Species Composition of Microplankton in the Coastal Waters of the East Coast of Peninsular Malaysia”. In Thirteenth Trawl Survey of the Coastal Waters off the East Coast of Peninsular Malaysia, Edited by: Mansor, M. I. 1–21. Kuala Lumpur, Malaysia: Ministry of Agriculture Malaysia.

APHA (American Public Health Association). 1998. Standard Methods for the Examination of Water and Wastewater, , 20th edition, Edited by: Lenore, S. C., Arnold, E. G. and Andrew, D. E. Washington D. C., USA: American Public Health Association.

Butler, P. A. 1963. “A review of fish and wildlife service investigations during 1961–1962”. In Commercial Fisheries Investigations, Pesticide-wildlife Series, Edited by: George, J. L. 11–25. Gulf Breeze, Florida, USA: US Department of the Interior, Fish and Wildlife Service.

Cheah, U. B. and Lum, K. Y. 1998. “Pesticide residue and microbial contamination of water resources in the Muda rice agroecosystem”. In Rice Agroecosystem of the Muda Irrigation Scheme, Malayisa, Edited by: Nashriyah, N. K., Ho, B. S., Ismail, A., Ali, B. and Lum, K. Y. 200–206. Kuala Lumpur, Malaysia: MINT-MADA.

Coutteau, P. and Sorgeloss, P. 1992. The requirement for live algae and their replacement by artificial diets in the hatchery and nursery rearing of bivalve mollusks: An international survey. J. Shellfish Res., 11: 467–476.

DeLorenzo, M. E., Taylor, L. A., Lund, S. A., Pennington, P. L., Strozier, E. D. and Fulton, M. H. 2002. Toxicity and bioconcentration potential of the agricultural pesticide endosulfan in phytoplankton and zooplankton. Arch. Environ. Contam. Toxicol., 42: 173–181.

Fujii, S., Nishimoto, N., Notoya, A. and Hellebust, J. A. 1995. Growth and osmoregulation of Chaetoceros muelleri in relation to salinity. Plant Cell Physiol., 36: 759–764.

Gandhi, S. R., Kulkarni, S. B. and Netrawali, M. S. 1988. Comparative effects of synthetic insecticides–endosulfan, phosalone and permethrin–on Chlamydomonas reinhardtii algal cells. Acta Microbiol. Hung., 35: 93–99.

Hii, Y. S., Lee, M. Y. and Chuah, T. S. 2007. Acute toxicity of organochlorine insecticide endosulfan and its effect on behaviour and some hematological parameters of Asian swamp eel (Monopterus albus, Zuiew). Pestic. Biochem. Physiol., 89: 46–53.

Kobraei, M. E. and White, D. S. 1996. Effects of 2,4-Dichlorophenoxyacetic acid on Kentuckey algae. Arch. Environ. Contam. Toxicol., 31: 571–580.

Lacerda, S. R., Koening, M. L., Neumann-Leitão, S. and Flores-Montes, M. J. 2004. Phytoplankton nyctemeral variation at a tropical river estuary. Braz. J. Biol., 64: 81–94.

Lassen, M. F., Bramm, M. E., Richardson, K., Yusoff, F. M. and Shariff, M. 2004. Phytoplankton community composition and size distribution in the Langat River Estuary. Malaysia, Estuaries, 27: 716–728.

Mackay, D., Shiu, W. Y., Lee, S. C. and Ma, K. 2006. Handbook of Physical-Chemical Properties and Environmental Fate for Organic Chemicals, Boca Raton, Florida, USA: CRC Press Inc..

Mohapatra, P. K. and Mohanty, R. C. 1992. Growth pattern changes of Chlorella vulgaris Anabaena doliolum due to toxicity of dimethoate and endosulfan. Bull. Environ. Contam. Toxicol., 49: 576–581.

Okay, O. S. and Gaines, A. 1996. Toxicity of 2,4-D acid to phytoplankton. Water Res., 30: 688–696.

Parsons, T. R., Malta, Y. and Lalli, C. M. 1984. A Manual of Chemical and Biological Method for Seawater Analysis, Oxford: Pergamon Press.

Rioboo, C., González, O., Herrero, C. and Cid, A. 2002. Physiological response of freshwater microalgae (Chlorella vulgaris) to triazine and phenylurea herbicides. Aquat. Toxicol., 59: 225–235.

Sandnes, J. M., Källqvist, T., Wenner, D. and Gislerød, H. R. 2005. Combined influence of light and temperature on growth rates of Nannochloropsis oceanica: linking cellular responses to large-scale biomass production. J. Appl. Phycol., 17: 515–525.

Sarma, Y. S. R. K. and Tripathi, S. N. 1980. Effects of some chemicals (2,4- dichlorophenoxyacetic acid, coumarin and acenaphthene) on the green alga, Oedogonium acmandrium Elfving. Phykos., 19: 142–152.

Schanz, F. and Zahler, U. 1981. Prediction of algal growth in batch culture. Schweiz. Z. Hydrol., 43: 103–113.

Smith, L. L., Fox, J. M. and Granvil, D. R. 1993. “Intensive algae culture techniques”. In CRC Handbook of Mariculture. Volume 1: Crustacean Aquaculture, Edited by: McVey, J. P. 3–13. Boca Raton, Florida: CRC Press Inc..

Srivastava, V. and Misra, P. C. 1981. Effect of endosulfan on plasma membrane function of the yeast Rhodotorula gracilis. Toxicol. Lett., 7: 475–480.

Steven, J. T. and Sunner, D. D. 1991. “Herbicides”. In Handbook of Pesticides Toxicology, Edited by: Hayes, W. J. and Laws, E. R. 1356–1376. San Diego, USA: Academic Press Inc..

Tu, M., Hurd, C. and Randall, J. M. 2001. Weed Control Methods Handbook. The Nature Conservancy, http://tncweedsucdavis.edu Version: April 2001

Wong, P. T. S. and Couture, P. 1986. “Toxicity screening using phytoplankton”. In Toxicity Testing Using Microorganisms, Vol. 2, Edited by: Dutka, B. J. and Biltton, G. 79–100. Boca Raton, Florida, USA: CRC Press Inc..

Zhou, H., Huang, X., Zhou, H. Q., Liu, X. G. and Yuan, C. 2003. (The effect of plant growth and regulation materials on protein and amino acids of algae in bait. In Chinese). Journal of Shanghai Fisheries University (China), 3: 215–218.

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Published

2009-11-30

Issue

Vol. 12 No. 4 (2009): Ecosystem sustainability & health of threatened marine environments (ESHTME)

Section

Research article

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