Nuisance Didymosphenia geminata blooms in the Argentinean Patagonia: Status and current research trends
Keywords:
diatom, invasions, Argentina, phylogeographyAbstract
Large nuisance blooms of Didymosphenia geminata have become increasingly widespread in Patagonia. Although the first published account for South America was in 1964, reports of large growths in Chile and Argentina commenced around 2010. Since then, these blooms have been observed all along the Andes region to the south of parallel 42°S. General surveys are needed to help provide an explanation. Possibilities include one or more new genetic variants or responses of local populations to global environmental changes. Electron microscopy of material from the Argentinean Patagonia revealed marked differences between regions, though it is unclear how much local factors and/or variations in life cycle contribute. Thus, we are approaching the problem from a molecular perspective, which we hope will help to overcome this limitation. Initial studies showed that D. geminata seems to be highly recalcitrant to DNA extraction, thus hindering the survey of molecular markers. We have now developed an improved DNA extraction technique for Didymosphenia mats, which markedly outperforms other techniques. However, endpoint polymerase chain reaction analyses suggest the persistence of polymerase chain reaction inhibitors in the samples, highlighting the need of further improvements for quantitative studies.
References
Abelli Bonardi, M., Baffico, G., Beamud, G., Castiñeira, L., Díaz, M., Laffitte, L., Laurenzano, B., Morzenti, P., Muñiz Saavedra, J., Pedrozo, F., Rivera, D., Temporetti, P., 2012. Programa de vigilancia, seguimiento y control de Didymosphenia geminata (Lyngbye) Schmidt 1899 en la Provincia del Neuquén. (Program of surveillance, monitoring and control of Didymosphenia geminata (Lyngbye) Schmidt 1899 in the Province of Neuquen. In Spanish.). Presented at: 5th Argentinean Congress of Limnology, 2012 November 28–December 1, Santa Fé, Argentina. Instituto Nacional de Limnología and Facultad de Humanidades y Ciencias, Universidad Nacional del Litoral.
Aboal, M., Marco, S., Chaves, E., Mulero, I., García-Ayala, A., 2012. Ultrastructure and function of stalks of the diatom Didymosphenia geminata. Hydrobiologia 695(1), 7–24.
Aljanabi, S.M., Martinez, I., 1997. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res. 25(22), 4692–4693.
Asprey, J.F., Benson-Evans, K., Furet, J.E., 1964. A contribution to the study of South American freshwater phytoplankton. Gayana: Botánica 10, 1–18.
Beamud, G., Baffico, G., Pedrozo, F., Diaz, M., 2013. First record of the invasive algae Didymosphenia geminata in the Lake Nahuel Huapi: Argentina, Patagonia. Rev. Chil. de Hist. Nat. 86(2), 493–496.
Bey, B.S., Fichot, E.B., Dayama, G., Decho, A.W., Norman, R.S., 2010. Extraction of high molecular weight DNA from microbial mats. Biotechniques 49(3), 631–640.
Biggs, B.J.F., Kilroy, C., Vieglais, C.C., 2006. A New Zealand science response to help manage Didymosphenia geminata – an unwanted diatom invader of freshwaters. In: S. Spaulding, R. Wiltshire, L. Elwell (Eds.), Current knowledge of Didymosphenia geminata: Developing a research and management response, p. 7. Federation of Fly Fishers and EPA Region 8, held in association with Western Division American Fisheries Society Annual Meeting, Montana State University, Montana, USA.
Blanco, S., Ector, L., 2009. Distribution, ecology and nuisance effects of the freshwater invasive diatom Didymosphenia geminata (Lyngbye) M. Schmidt: a literature review. Nova Hedwigia 88(3–4), 347–422.
Cary, S.C., Hicks, B.J., Crawfod, N.J., Coyne, C., 2006. A sensitive genetic based detection capability for Didymosphenia geminata, Interim CBER Contract Report 45, prepared for MAF Biosecurity New Zealand, Centre for Biodiversity and Ecology Research, The University of Waikato.
Cary, S.C., Hicks, B.J., Coyne, K.J., Rueckert, A., Gemmill, C.E.C., Barnett, C.M.E., 2007. A sensitive genetic-based detection capability for Didymosphenia geminata (Lyngbye) M. Schmidt: Phases Two and Three, CBER Contract Report 62, prepared for MAF Biosecurity New Zealand, Centre for Biodiversity and Ecology Research, The University of Waikato.
Cary, S.C., Coyne, K.J., Rueckert, A., Wood, S.A., Kelly, S., Gemmill, C.E.C., Vieglais, C., Hicks, B.J., 2014. Development and validation of a quantitative PCR assay for the early detection and monitoring of the invasive diatom Didymosphenia geminata. Harmful Algae 36, 63–70.
Clement, M., Posada, D., Crandall, K.A., 2000. TCS: a computer program to estimate gene genealogies. Mol. Ecol. 9(10), 1657–1659.
Cota Sanchez, J.H., Remarchuk, K., Ubayasena, K., 2006. Ready-to-Use DNA Extracted with a CTAB Method Adapted for Herbarium Specimens and Mucilaginous Plant Tissue. Plant Mol. Biol. Rep. 24, 161–167.
Ellwood, N.T.W., Whitton, B.A. 2007. Importance of organic phosphate hydrolyzed in stalks of the lotic diatom Didymosphenia geminata and the possible impact of climatic and atmospheric changes. Hydrobiologia 592(1), 121–133.
Evans, K.M., Wortley, A.H., Mann, D.G., 2007. An assessment of potential diatom “barcode” genes (cox1, rbcL, 18S and ITS rDNA) and their effectiveness in determining relationships in Sellaphora (Bacillariophyta). Protist. 158(3), 349–364.
Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O., 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59(3), 307–321.
Jackson, D.P., Lewis, F.A., Taylor, G.R., Boylston, A.W., Quirke, P., 1990. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J. Clin. Pathol. 43(6), 499–504.
Jackson, L.J., Corbett, L., Scrimgeour, G., 2016. Environmental constraints on Didymosphenia geminata occurrence and bloom formation in Canadian Rocky Mountain lotic systems. Can. J. Fish. Aquat. Sci. 73(6), 964–972.
James, D.A., Chipps, S.R., 2016. Influence of Didymosphenia geminata Blooms on Prey Composition and Associated Diet and Growth of Brown Trout. T. Am. Fish. Soc. 145(1), 195–205.
Jaramillo, A., Osman, D., Caputo, L., Cardenas, L., 2015. Molecular evidence of a Didymosphenia geminata (Bacillariophyceae) invasion in Chilean freshwater systems. Harmful Algae 49, 117–123.
Jones, H.M., Simpson, G.E., Stickle, A.J., Mann, D.G., 2005. Life history and systematics of Petroneis (Bacillariophyta), with special reference to British waters. Eur. J. Phycol. 40(1), 61–87.
Jones, L.R., Uyua, N.M., Manrique, J.M., 2015. The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata. Biodivers. Conserv. 24(6), 1541–1548.
Kermarrec, L., Ector, L., Bouchez, A., Rimet, F., Hoffmann, L., 2011. A preliminary phylogenetic analysis of the Cymbellales based on 18S rDNA gene sequencing. Diatom Res. 26(3), 305–315.
Khan-Bureau, D.A., Morales, E.A., Ector, L., Beauchene, M.S., Lewis, L.A., 2016. Characterization of a new species in the genus Didymosphenia and of Cymbella janischii (Bacillariophyta) from Connecticut, USA. Eur. J. Phycol. 51(2), 203–216.
Kilroy, C., Bothwell, M.L., 2011. Enviromental control of stalk length in the bloom-forming, freshwater benthic diatom Didymosphenia geminata (Bacillariopyceae). J. Phycol. 47(5), 981–989.
Manrique, J.M., Calvo, A.Y., Halac, S.R., Villafane, V.E., Jones, L.R., Walter Helbling, E., 2012. Effects of UV radiation on the taxonomic composition of natural bacterioplankton communities from Bahia Engano (Patagonia, Argentina). J. Photochem. Photobiol. B. 117, 171–178.
Montecino, V., Molina, X., Bothwell, M., Muñoz, P., Carrevedo, M.L., Salinas, F., Kumar, S., Castillo, M.L., Bizama, G., Bustamante, R.O., 2016. Spatio temporal population dynamics of the invasive diatom Didymosphenia geminata in central-southern Chilean rivers. Sci. Total Environ. 568, 1135–1145.
Moon-van der Staay, S.Y., De Wachter, R., Vaulot, D., 2001. Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature 409(6820), 607–610.
Nakov, T., Ruck, E.C., Galachyants, Y., Spaulding, S.A., Theriot, E.C., 2014. Molecular phylogeny of the Cymbellales (Bacillariophyceae, Heterokontophyta) with a comparison of models for accommodating rate variation across sites. Phycologia 53(4), 359–373.
Nylander, J.A., 2004. MrAIC.pl, Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Porebski, S., Bailey, L.G., Baum, B.R., 1997. Modification of a CTAB DNA Extraction Protocol for Plants Containing High Polysaccharide and Polyphenol Components. Plant Mol. Biol. Rep. 15(1), 8–15.
Rivera, P., Gebauer, M., 1989. Diatomeas chilenas en las Colecciones de Boyer, Cleve & Moeller, Schulze y Smith, depositadas en la Academia de Ciencias Naturales de Filadelfia, Estados Unidos. (Chilean diatoms in the collections of Boyer, Cleve & Moeller, Schulze and Smith, deposited in the Academy of Natural Sciences, Philadelphia, USA. In Spanish.). Gayana Botanica 46, 89–116.
Rivera, P., Basualto, S., Cruces, F., 2013. Acerca de la diatomea Didymosphenia geminata (Lyngbye) M. Schmidt: su morfología y distribución en Chile. About the diatom Didymosphenia geminata (Lyngbye) M. Schmidt: Their morphology and distribution in Chile. In Spanish). Gayana Botanica 70, 154–158.
Rogers, S.O., Bendich, A.J., 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant. Mol. Biol. 5(2), 69–76.
Sastre, A.V., Santinelli, N.H., Bauer, G.A., Ayestarán, M.G., Uyua, N.M., 2013. First record of the invasive diatom Didymosphenia geminata (Lyngbye) Schmidt in a Patagonian Andean river of Argentina. Bioinvasions Rec. 2(1), 11–17.
Segura, P., 2011. Ecology. A slimy invader blooms in the rivers of Patagonia. Science. 331(6013), 18.
Taylor, B. W., Bothwell, M. L., 2014. The Origin of Invasive Microorganisms Matters for Science, Policy, and Management: The Case of Didymosphenia geminata. BioScience, 64(6), 531–538.
Uyua, N.M., Manrique, J.M., Jones, L.R., 2014. An optimized DNA extraction protocol for benthic Didymosphenia geminata. J. Microbiol. Met. 104C, 12–18.
Uyua, N.M., Santinelli, N.H., Sastre, A.V., Sala, S.E., 2016. Morfología valvar de Didymosphenia geminata (Bacillariophyceae) en la provincia de Chubut, Argentina. Bol. Soc. Argent. Bot. 51(1), 15–27.
Watson, S.B., Whitton, B.A., Higgins, S.N., Paerl, H.W., Brooks, B.W., Wehr, J.D., 2015. Harmful algal blooms. In J.D. Wehr, R.G. Sheath, R.P. Kociolek (Eds.), Freshwater Algae of North America: Classification and Ecology. 2nd Edition. Academic Press, Massachusetts.
Weinbauer, M.G., Fritz, I., Wenderoth, D.F., Höfle, M.G., 2002. Simultaneous extraction from bacterioplankton of total RNA and DNA suitable for quantitative structure and function analyses. App. Environ. Microbiol. 68 (3), 1082–1087.
Whitton, B.A., Ellwood, N.T.W., Kawecka, B., 2009. Biology of the freshwater diatom Didymosphenia: a review. Hydrobiologia 630(1), 1–37.
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