Spatial analysis of bacterioneuston and bacterioplankton diversity in the north China Sea

Authors

  • Jingfeng Fan Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
  • Hongjun Li Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
  • Yongkai Chang Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
  • Xiaohui Wang Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
  • Hongxia Ming Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
  • Jie Su Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China
  • Hongbo Li Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China

Keywords:

sea surface microlayer, subsurface layer, bacteria, abundance, community, denaturing gradient gel electrophoresis

Abstract

In this study, we investigated the bacterial diversity in the surface microlayer (<1 mm) and its corresponding subsurface layer (0.5 m in depth) and compared their bacterial communities in seven sites located across the northern Yellow Sea and Bohai Sea in China. Bacterial cell abundance was determined by flow cytometry; the structure of the bacterial assemblage was quantitatively assessed through denaturing gradient gel electrophoresis. Bacterial cell abundant was not significantly different between bacterioneuston and bacterioplankton. The colony-forming units in the surface microlayer were significantly enriched compared with that in the subsurface layer. Enrichment factors (the ratio of abundance in the surface layer to that in the subsurface layer) led to high total cell counts in the surface microlayer of the cultivated bacteria (46.92 and 1.28 for colony-forming units and cells, respectively). The fraction of neustonic bacteria was similar among the sites, despite large differences in environmental conditions. Moreover, the community structures of bacterioneuston and bacterioplankton exhibited distinct and consistent differences.

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Published

2018-01-02

Issue

Vol. 21 No. 1 (2018)

Section

Research article

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