Removal of Anabaena sp. bloom and microcystin-LR by coculturing with Mucor rouxii pellets

Authors

  • Ayad M.J. Al-Mamoori University of Babylon, College of Science, Biology Dept. Baghdad, Iraq
  • Rana H. H. Al-Shammari Mustansiriyah University, College of Science, Biology Baghdad, Iraq
  • Moayed J.Y. Al-amari University of Babylon, College of Science, Biology Dept. Baghdad, Iraq
  • Maher M. K. Al-Juboori University of Babylon, College of Science, Biology Dept. Baghdad, Iraq

Keywords:

biocontrol, harmful algae, coculture, fungal pellets

Abstract

In this study, biocontrol of harmful effect of cyanobacterial blooms and their toxins by “flocculation-biosorption” was achieved. Five fungal species were isolated from decayed cyanobacterial bloom which are: Aspergillus fumigatus, A. niger, Penicillium, Trichoderma ressei and Mucor rouxii. We chose the last species’ pellets because they are the most stable and cocultured with Anabeana sp. (1:5 fungal: cyanobacteria ratio) of dry weight, Harvest Efficacy HE% by fungal pellets started after 12h of co-culturing about (4%) and almost complete harvesting after 48h with (98%), then we add 0.1g of Magnetite nano Fe3o4 to facilitate removing cyanobacterial blooms. Microcystin-LR extracted from Anabaena sp. were purified and collected by preparative high-performance liquid chromatography (HPLC) was 75.1 (µg ml-1), M. rouxii pellet absorbed about 85% of Microcystin-LR after 72 h of incubation at 25 °C.

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Published

2020-07-02

Issue

Vol. 23 No. 3 (2020): Aquatic Ecosystem Health & Management

Section

Research article

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